Cyclin-dependent kinase (CDK) inhibitor Roscovitine proliferating PC12 cells led to the death of nature and the CDK regulation of the cell cycle and cell apoptosis. Method using cultured PC12 cells proliferative phase model for MTT cell activity, Hoechst 33342 staining nuclei and inverted microscope. The results were used to 5,10,20,50 and 100μmol / L solution of the concentration of cells Roscovitine 12h, showed a dose-dependent decrease in cell viability; Further, 50μmol / L concentration Roscovitine 12 and 24 cells were treated and 48h, also showed time-dependent cell death. Morphological manifestations of cell death cell shrinkage, nuclear chromosome condensation and nuclear fragmentation formation. Conclusion Roscotivine proliferating PC12 cells induced apoptosis and regulation of cyclin CDK relevant.
Roscovitine stimulate proliferation of PC12 cells
ReplyDeleteCyclin-dependent kinase (CDK) inhibitor Roscovitine proliferating PC12 cells led to the death of nature and the CDK regulation of the cell cycle and cell apoptosis. Method using cultured PC12 cells proliferative phase model for MTT cell activity, Hoechst 33342 staining nuclei and inverted microscope. The results were used to 5,10,20,50 and 100μmol / L solution of the concentration of cells Roscovitine 12h, showed a dose-dependent decrease in cell viability; Further, 50μmol / L concentration Roscovitine 12 and 24 cells were treated and 48h, also showed time-dependent cell death. Morphological manifestations of cell death cell shrinkage, nuclear chromosome condensation and nuclear fragmentation formation. Conclusion Roscotivine proliferating PC12 cells induced apoptosis and regulation of cyclin CDK relevant.
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