PD98059, U0126 on hepatoma MAPK and JAK-STAT signaling pathways
Observation PD98059, U0126 on hepatoma SMMC-7721 cells proliferation and MAPK, JAK-STAT pathway. Methods MEK pathway inhibitor PD98059, U0126 effect on liver cancer cell SMMC-7721, by MTT assay at different concentrations PD98059, U0126 on SMMC-7721 cell proliferation; RT-PCR assay PD98059, U0126 on SMMC-7721 cells erk, stat3, stat5 mRNA influence, Western blot assay PD98059, U0126 on hepatoma SMMC-7721 cells ERK, STAT3, STAT5, P-ERK, P-STAT3, P-STAT5 protein expression. Results PD98059, U0126 can inhibit the proliferation of SMMC-7721 cells in a time - dose dependent. PD98059, U0126 effect on SMMC-7721 cells, erk, stat3, stat5 mRNA expression compared with the control group, the difference was not statistically significant (P005). P-ERK, P-STAT3, P-STAT5 protein expression compared with the control group, the difference was significant (P0.05). Conclusion PD98059, U0126 reduced ERK, STAT3, STAT5 phosphorylation levels of protein kinases, thereby inhibiting tumor cell proliferation.
PD98059, U0126 on hepatoma MAPK and JAK-STAT signaling pathways
ReplyDeleteObservation PD98059, U0126 on hepatoma SMMC-7721 cells proliferation and MAPK, JAK-STAT pathway. Methods MEK pathway inhibitor PD98059, U0126 effect on liver cancer cell SMMC-7721, by MTT assay at different concentrations PD98059, U0126 on SMMC-7721 cell proliferation; RT-PCR assay PD98059, U0126 on SMMC-7721 cells erk, stat3, stat5 mRNA influence, Western blot assay PD98059, U0126 on hepatoma SMMC-7721 cells ERK, STAT3, STAT5, P-ERK, P-STAT3, P-STAT5 protein expression. Results PD98059, U0126 can inhibit the proliferation of SMMC-7721 cells in a time - dose dependent. PD98059, U0126 effect on SMMC-7721 cells, erk, stat3, stat5 mRNA expression compared with the control group, the difference was not statistically significant (P005). P-ERK, P-STAT3, P-STAT5 protein expression compared with the control group, the difference was significant (P0.05). Conclusion PD98059, U0126 reduced ERK, STAT3, STAT5 phosphorylation levels of protein kinases, thereby inhibiting tumor cell proliferation.
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